Solid-phase synthesis is highly accurate, however, is an expensive process and offers low output. Chip-based DNA synthesis is less expensive than solid-phase method and can produce a large amount of the target gene, however, with less accuracy. PCR-based enzyme synthesis produces gene fragments with a variety of cell systems. It synthesizes long gene fragments with higher accuracy. Gene synthesis methods have a profound impact in vaccine design, genetic and metabolic engineering. However, currently available methods are inconsistent with drawbacks such as high error rate, low yield, and lack of scalability on the size of the gene and size of the project.
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