In both fundamental and applied sciences, cell-free protein expression is a crucial tool for molecular biologists. Researchers working on high-throughput functional proteomics and genomics are increasingly using cell-free protein expression because it offers several benefits over protein production in live cells. For enzyme engineering employing display technologies and the creation of protein arrays, such as nucleic acid programmable protein arrays, cell-free protein expression is important (NAPPA). A DNA template (which might be a plasmid or a polymerase chain reaction product) encoding the desired protein is needed for cell-free protein production, along with a solution containing all the enzymes and transcription and translation factors that are required.
This solution is a cell-free extract since the cell membrane has been removed, leaving just the organelle and cytosol. Expression of the target protein might begin when the genetic material and the cell-free extract are mixed together.
By 2030, the market for cell-free protein expression will surpass US$ 459.9 million.
The market for Cell-Free Protein Expression is anticipated to reach US$ 280.2 million in 2022 and grow at a 6.4% CAGR during the forecast period (2022-2030).
Key Leaders in the Cell-Free Protein Expression Industry:
1. Thermo Fisher Scientific Inc.
An American provider of scientific equipment, reagents, and consumables as well as software services is Thermo Fisher Scientific Inc. Thermo Fisher, headquartered in Waltham, Massachusetts, was created in 2006 by the union of Thermo Electron and Fisher Scientific. To address difficult analytical issues in research, diagnostics, and clinical laboratories, it is a provider of medical equipment, analytical instruments, reagents and consumables, software, and services. It provides answers for flow cytometry, synthetic biology, and cellular biology. The firm offers a variety of goods, including as technology for protein and molecular biology research, electron microscopy, mass spectrometry, and genetic sequencing. The business provides services to academic and research institutions, government agencies, pharmaceutical and biotech firms, clinical diagnostic laboratories, environmental, industrial quality, and process control sectors.Throughout the Americas, Asia-Pacific, and Europe, it offers its goods and services through its direct sales force, online sales, and third-party distributors. Waltham, Massachusetts, in the US, is home to Thermo Fisher's headquarters.
2. Takara Bio inc
Takara Bio USA, Inc., which was established in April 2002, offers kits, reagents, tools, and services to support study into topics relating to gene discovery, regulation, and function. Kusatsu, Shiga, Japan, is home to its headquarters. The business provides inducible systems, fluorescent proteins and reporters, cloning and competent cells, kits, reagents, and cell and molecular biology. The global operations of Clontech Labs. In order to imitate type 2 diabetes in a susceptible population, researchers created pancreatic beta cell-like cells from patient-specific tissue in 2023.
3. Promega Corporation
The 1978-founded Promega Corporation is a producer of enzymes and other products for biotechnology and molecular biology with headquarters in Madison, Wisconsin. The company's product line includes products for genomics, protein analysis and expression, cellular analysis, drug discovery, and genetic identity. The 2,000 products produced by Promega Corporation help scientists all around the world expand their understanding of life science research, notably in the fields of genomics, proteomics, and cellular analysis. The firm has between 1001 and 5000 workers and is based in the Madison, WI region.
4. New england biolabs
New England Biolabs, established in 1974, manufactures and distributes native and recombinant enzyme reagents for use in life science research, as well as offering goods and services to enable genome editing, synthetic biology, and next-generation sequencing. Research tools like REBASE, InBASE, and Polbase are also accessible without charge through NEB. With 350 workers and $43.0M in yearly revenue, Massachusetts-based New England Biolabs is a medium-sized healthcare organisation.
5. Moderna therapeutics
Moderna Inc. (Moderna), a company that was established in September 2010, is a messenger RNA therapies developer. The business creates and discovers medications that cause patient cells to manufacture proteins or antibodies. Its drug development pipeline comprises a range of therapeutic and preventative vaccinations, localised therapies, intratumoral immuno-oncology, and liver intracellular vaccines. Moderna creates and generates human proteins, antibodies, and new proteins using their mRNA therapies platform, aiding in the diagnosis, treatment, and prevention of illnesses. Moreover, it creates treatments for cardiovascular illness, rare liver diseases, infectious diseases, and immuno-oncology. Cambridge, Massachusetts, in the US, serves as the corporate headquarters of Moderna Therapeutics. For the prevention of COVID-19, the vaccine, formerly known as the Moderna COVID-19 Vaccine, will now be sold as Spikevax.
6. Merck KGaA
The Merck Group, founded in 1668 and marketed as Merck, is a worldwide scientific and technology firm with its headquarters in Darmstadt with operations in 66 different countries. Almost 250 businesses make up the organisation, with Merck KGaA in Germany serving as its centrepiece. A division of E. Merck KG is the scientific and technology firm Merck KGaA (Merck). It also creates liquid crystal combinations, organic light-emitting diode (OLED) materials, cosmetic active ingredients, coating pigments, and high-tech materials. These medications are prescribed to treat cancer, multiple sclerosis, and infertility. Moreover, Merck offers a wide range of goods, such as end-to-end systems, cell lines, antibodies, lab water systems, gene editing tools, and tools for editing genes. The business provides services to the life sciences, performance materials, and healthcare sectors.It is present throughout the Middle East, Africa, Latin America, North America, and Asia-Pacific. The headquarters of Merck are in Darmstadt, Hesse, Germany.
7. Creative Biolabs Inc.
In 2015, Creative Biolabs Inc. was established. The corporation conducts commercial research and development in the physical and biological sciences. Using pre-made libraries, phage display services, different phage display libraries, antibody sequencing, and antibody humanization are just a few of the biotech goods and services that Creative Biolabs, Inc. manufactures and provides for early drug discovery and development.
8. Biotechrabbit
In 2011, Biotechrabbit was established. The company's primary goal is to produce enzymes and antibodies for diagnostic firms. High-quality, ultra-pure proteins, reagents, and kits are offered by the business for both standard and unique formulations. Its main office is located in Hennigsdorf, Berlin (Germany).
1. Biotechrabbit offers a variety of services, such as:
2. custom monoclonal and polyclonal antibodies and fermentation over 1 kg
3. production of proteins and enzymes in E. coli and mammalian cell lines
4. assay development
5. contract research
6. enhancement of enzymes using rational and irrational molecular approaches
7. protein expression in vitro, Rapid Translation System - RTS
8. mRNA service
*Definition - Cell-free protein expression solution is a cell-free extract, as it consists only the organelle and cytosolic material while the cell membrane has been removed.
